Particle Tracking Analysis (PTA) with ZetaView®
01.07.2014

  • Theory
    • 90° light scattering set-up
    • Brownian motion hydrodynamic size, electrophoresis, zeta potential, concentration, size range, concentration range

  • Mechanical design
    • Scattering volume, minimum sample requirement
    • Minimum 1 sub-volume scan for truendess and high reproducibility of the results
    • Cell cassette design: Peltier, fluidics, easy cell change and cleaning
    • Lasers and filters
    • Light scattering and fluorescent mode

  • Software
    • Autoalignment
    • The importance of the correct camera settings
    • Statistical approach for the selection of acquired results

  • Application examples
    • Gold, silver
    • Liposomes, proteins
    • Clinical samples: Exosomes of various origin (tumor, aging, dermatology, cell culture, serum)

  • Limits and strengths of the ZetaView PTA
  • Complementarity with 180° DLS results
  • Final statement
  • Video of operation
  • Q&A

The Duo Stabino / NANO-flex
26.03.2014