Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are in the growing interest of the biosciences and nanomedicine. With the NTA technology, particles are individually measurable in a physiological buffer and you observe what you measure in a video. A new step forward in convenience and speed for fluorescence studies of EV-antibody conjugates has been achieved with the ZetaView® QUATT Lasers and fluorescence filters are pre-selectable via the software. This procedure saves time and sample.

Typical application

Determining the absolute concentration is one of the strengths of the NTA technique. In scatter mode, the concentration of all particles is measured. Changing subsequently to the fluorescence mode, marked biologic nanoparticles are tracked. By using four lasers within a SOP assisted sequence, it is possible to differentiate between four different fluorescence dyes in the same sample. Phenotyping of tetraspanins for instance, on vesicles and virus is facilitated, positive and isotype control as well. To prepare instrument settings, standard EVs may be used (HBM HansaBioMed https://www.hansabiomed.eu/).

 

ZetaView® NTA Simulator

 

  • very short measuring times
  • "Ready to use" calibration standard
  • 11 measuring positions, optionally combined with dosing steps
  • Outlier free result
  • sample recovery
  • Low Bleach Technology
  • Fully automatic focusing of the optics
  • Quartz cell channel and easy cleaning
  • SOP methodology
  • Applicable in the GMP range
  • IQ / OQ / PQ

  • 4 fluorescence channels per sample
  • Laser and fluorescence channels switched per mouse click
  • Upgradable from PMX-120 BASIC to PMX-220 TWIN or PMX-420 QUATT
  • Choice of different fluorescence settings per SOP
  • 4 lasers installed with the wavelengths
    • 405 nm , 488 nm, 520 nm, 640 nm

Proof of Principle with 4 antibodies and vesicle markers with example CD9, CD41, CD63, CD81 and markers CMG, CMDR.

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Operating ZetaView®

11 Positions Scan


To capture many individual particles in the field of view, the ZetaView® optic scans a wide field of the cell channel. In addition, additional sample volume is automatically added by means of a precise pump.

Nanoparticle Tracking Analysis (NTA) with ZetaView®

Brownian Diffusion in the Laser Scattering Video Microscope

120 nm Al203 particles and agglomerates in the electrophoresis set-up of ZetaView

Nanoparticle Tracking Analysis (NTA) with the Particle Metrix ZetaView® instrument family allows fast and reliable measurement of size, concentration, and Zeta Potential of nanoparticles. In contrast to scatter-based particle detection, fluorescence detection capability of the ZetaView® instrument allows the user to get biochemical information about particles. This increases the resolution and discriminatory power of the data. Furthermore, our recently introduced “Multi Filter Feature” allows Fluorescence Nanoparticle Tracking Analysis performing multi fluorescence with only one laser.  Thus, two different fluorescence dyes can be detected with one wavelength making F-NTA in the ZetaView® instrument more versatile ever since.

Get the newest updates of biomarker detection with NTA:

  • Release of multiple filter upgrade: Double staining with ONE laser!
  • Optimization of labeling strategies
  • Release of FDA 21 CFR part 11 software

Nanoparticle Tracking Analysis (NTA) with the Particle Metrix ZetaView® instrument family allows fast and reliable measurement of size, concentration, and Zeta Potential of nanoparticles in the size range from 10 nm to 2 µm. On basis of the scatter data, the user cannot recognize and distinguish whether the particles are biological vesicles, protein aggregates, inorganic precipitates or simply cell waste. However, the fluorescence detection capability of the ZetaView® instrument allows the user to get biochemical information about particles, thus increasing the resolution and discriminatory power of the data. In this video we describe the cutting-edge measurement technology of the ZetaView® instrument family and present the latest f-NTA results on extracellular vesicle phenotyping using membrane dyes and monoclonal antibodies.