Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.

Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.

Very short measuring time:
With the Zetaview® you can measure more than 1000 particles within 90 seconds.

Robust statistics with outlier control
11 different positions within the measuring cell yield statistically robust results compared to only one measuring position. The implemented outlier control detects artifacts in the video quality and only considers flawless videos.

Save time by focusing the optics and during functional testing
For the "AutoAlignment" and the daily quality test, there is a "ready to use" 99 nm reference standard in the measurement concentration of 2.1x107 / ml, filled in a 3 ml syringe.

SOPs for pre-programmed work
Presets of measurement parameters such as size and surface charge are adjusted to scatter- or fluorescence mode, respectively.

Robust and contrast-optimized quartz glass measuring cell
The quartz glass makes the measuring cell durable and robust for cleaning procedures while maintaining high contrast for clear video.

Easy sample injection
The injection takes place with commercial syringes. The optimal measurement concentration is 2x107 particles / ml. Depending on the sample, typical dilutions are from 1:10 to 1: 10,000.

Sample recovery
The NTA measuring cell unit requires at least 600 μl sample. The sample can be recovered after the measurement without further effort,

ZetaView® fits in a pilot case and is easy to carry
That's what our sales and support people love, and of course our customers.

Fluorescence and photostability
The timing of the laser is optimized for minimal bleaching of low-lifetime fluorophores. Nevertheless, selecting stable fluorophores for the analysis of conjugated extracellular vesicles is advantageous. The ability to detect less than 1 fluorescent particle per 10,000 non-fluorescent increases significantly.

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Ye Zhao, Juan Xu Synovial fluid-derived exosomal lncRNA PCGEM1 as biomarker for the different stages of osteoarthritis. International Orthopaedics (SICOT) (2018). doi.org/10.1007/s00264-018-4093-6Abstract

Peter E. Petrochenko, Naresh Pavuralab, Yong Wu, Sook Yee Wong, Hamideh Parhiz et al. Analytical considerations for measuring the globule size distribution of cyclosporine ophthalmic emulsions. International Journal of Pharmaceutics Volume 550, Issues 1–2, 25 October 2018, Pages 229-239Abstract

Chun-Jung Lin, Andrew Dang, Elizabeth Hernandez, Jer-Tsong Hsieh Caveolin-1 detected in tumor-derived exosomes induces cancer stem cell phenotypes in castration-resistant prostate cancer. Proceedings of the AACR Special Conference: Prostate Cancer: Advances in Basic, Translational, and Clinical Research; 2017 Dec 2-5; Orlando, Florida. Philadelphia (PA): AACR; Cancer Res 2018;78(16 Suppl):Abstract nr A080Abstract

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F Rohn, C Kordes, M Castoldi, S Götze, G Poschmann et al. Laminin-521 promotes quiescence in isolated stellate cells from rat liver. Biomaterials, Available online 7 July 2018, In Press, Accepted Manuscript doi.org/10.1016/j.biomaterials.2018.07.008Abstract

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EHC Wong, YY Dong, M Coray, M Cortada, S Levano et al. Inner ear exosomes and their potential use as biomarkers. PLoS ONE 13(6): e0198029 Full text

G Jia, Y Han, Y An, Y Ding, C He, X Wang, Q Tang. NRP-1 targeted and cargo-loaded exosomes facilitate simultaneous imaging and therapy of glioma in vitro and in vivo. Biomaterials Volume 178, September 2018, Pages 302-316 Abstract

Bin Zhao, Xiaodong Li, Xiaomin Shi, Xueqin Shi, Wei Zhang, Gaofeng Wu, Xujie Wang, Linlin Su, and Dahai Hu. Exosomal microRNAs derived from human amniotic epithelial cells accelerate wound healing by promoting the proliferation and migration of fibroblasts. Research Article Full text

G Zhao, C Yang, J Yang, P Liu, K Jiang, A Shaukat et al. Soluble E-cadherin promotes tumor angiogenesis and localizes to exosome surface. Nature Communications volume 9, Article number: 2270 (2018) Full text

MKS Tang, PYK Yue, PP Ip, RL Huang, HC Lai et al. Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy. The Cell Surface, Volume 2, June 2018, Pages 14-23 Full text

J Rodrigues, CL Ramos, S Frases, RMC Godinho et al. Lack of chitin synthase genes impacts capsular architecture and cellular physiology in Cryptococcus neoformans. Cell Death & Disease volume 9, Article number: 704 (2018) Abstract

X Gao, N Ran, X Dong, B Zuo, R Yang, Q Zhou et al. Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy. Science Translational Medicine, 06 Jun 2018: Vol. 10, Issue 444, eaat0195, DOI: 10.1126/scitranslmed.aat0195 Abstract

RC Lai, RWY Yeo, B Zhang, M Koh, SS Tan et al. Topical Application of Yolk Lecithin Liposomes Reinforces Skin Barrier Function Against Chemical Agents Such as Psoriasis-inducing IMQ and Alleviates Disease Phenotype. Clin Exp Dermatol Ther: CEDT-146. DOI: 10.29011/2575-8268/100046 Full text

HA Scheidt, K Kolocaj, J Veje Kristensen, D Huster, D Langosch. Transmembrane Helix Induces Membrane Fusion through Lipid Binding and Splay. J. Phys. Chem. Lett., 2018, 9 (12), pp 3181–3186, DOI: 10.1021/acs.jpclett.8b00859 Abstract

AM da R Rondon, VH de Almeida, T Gomes et al. Low cardiorespiratory fitness is associated with higher extracellular vesicle counts in obese adults. Physiol Rep, 6 (10), 2018, e13701, doi.org/10.14814/phy2.13701Abstract

NZM Eichner, NM Gilbertson, JM Gaitan, EM Heiston et al. Tissue factor mediates microvesicles shedding from MDA-MB-231 breast cancer cells. Biochemical and Biophysical Research Communications, Volume 502, Issue 1, 7 July 2018, Pages 137-144 Full text

C DeMarino, ML Pleet, M Cowen, RA Barclay et al. Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1-Infected Cells. Scientific Reports volume 8, Article number: 7653 (2018) Full text

Sang June Park, Hyungtaek Jeon. Seung-Min Yoo, Myung-Shin Lee. The effect of storage temperature on the biological activity of extracellular vesicles for the complement system. In Vitro Cellular & Developmental Biology – Animal, June 2018, Volume 54, Issue 6, pp 423–429 Abstract

F Kunz, E Kontopoulou, B Giebel, N Neuhoff von, D Reinhardt, BK Thakur. Biomarker potential of mRNA in extracellular vesicles in pediatric AML. Klin Padiatr 2018; 230(03): 165, DOI: 10.1055/s-0038-1644986 Abstract

S Strachan, E Kontopoulou, E Bosch-Boras, D Reinhardt, BK Thakur. Exosomal (exo) dsDNA as a diagnostic and prognostic tool in pediatric solid tumours. Klin Padiatr 2018; 230(03): 169, DOI: 10.1055/s-0038-1645002 Abstract

S Chettimada, DR Lorenz, V Misra, ST Dillon et al. Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy. Scientific Reportsvolume 8, Article number: 7227 (2018) Full text

D Li, M Deng, Z Yu, W Liu, G Zhou, W Li, X Wang et al. Biocompatible and Stable GO-Coated Fe3O4 Nanocomposite: A Robust Drug Delivery Carrier for Simultaneous Tumor MR Imaging and Targeted Therapy. ACS Biomater. Sci. Eng., 2018, 4 (6), pp 2143–2154, DOI: 10.1021/acsbiomaterials.8b00029 Abstract

X Dai, C Chen, Q Yang, J Xue, X Chen, B Sun, F Luo et al. Exosomal circRNA_100284 from arsenite-transformed cells, via microRNA-217 regulation of EZH2, is involved in the malignant transformation of human hepatic cells by accelerating the cell cycle and promoting cell prolife. Cell Death & Disease, volume 9, Article number: 454 (2018) Full text

P Munson, YW Lam, M MacPherson, S Beuschel, A Shukla. Mouse serum exosomal proteomic signature in response to asbestos exposure. Journal of Cellular Biochemistry, September 2017, DOI: 10.1002/jcb.268 Full text

L Simón-Gracia, P Scodeller, SS Fuentes, V Gómez et al. Application of polymersomes engineered to target p32 protein for detection of small breast tumors in mice. Oncotarget, 2018, Vol. 9, (No. 27), pp: 18682-18697 Full text

Kwahun Lee, Liuyang Zhang Yi Yi, Xianqiao Wang, and Yan Yu. Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles. ACS Nano, 2018, 12 (4), pp 3646–3657, DOI: 10.1021/acsnano.8b00759 Abstract

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Shah N, Ishii M, Brandon C, Ablonczy Z, Cai J, Liu Y, Chou CJ, Rohrer B. Extracellular vesicle-mediated long-range communication in stressed retinal pigment epithelial cell monolayers. Biochimica et biophysica acta. 2018; 1864(8):2610-2622. PubMed [journal] PMID: 29684588 PubMed

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Tan, E., Asada, H.H. & Ge, R. Extracellular vesicle-carried Jagged-1 inhibits HUVEC sprouting in a 3D microenvironment. Angiogenesis (2018). doi.org/10.1007/s10456-018-9609-6Abstract

Xiao-fen Ruan, Cheng-wei Ju, Yan Shen, Yu-tao Liu, Il-man Kim, Hong Yu, Neal Weintraub, Xiao-long Wang & Yao-liang Tang. Suxiao Jiuxin pill promotes exosome secretion from mouse cardiac mesenchymal stem cells in vitro. Acta Pharmacologica Sinica doi:10.1038/aps.2018.19 Abstract

Xiao-fen Ruan, Yong-jun Li, Cheng-wei Ju, Yan Shen, Wei Lei, Can Chen, Yang Li, Hong Yu, Yu-tao Liu, Il-man Kim, Xiao-long Wang, Neal L Weintraub & Yao-liang Tang. Exosomes from Suxiao Jiuxin Pill-treated cardiac mesenchymal stem cells decrease H3K27 demethylase UTX expression in mouse cardiomyocytes in vitro. Acta Pharmacologica Sinica doi:10.1038/aps.2018.18 Abstract

YANG Ting Ting, LIU Chen Geng, GAO Shi Chao, ZHANG Yi, WANG Pei Chang. The Serum Exosome Derived MicroRNA-135a, -193b, and-384 Were Potential Alzheimer's Disease Biomarkers. Biomedical and Environmental Sciences, 2018, 31(2): 87-96. DOI: 10.3967/bes2018.011 Full text

Amrita Datta Chaudhuri, Raha M. Dastgheyb, Seung-Wan Yoo, Amanda Trout, C. Conover Talbot Jr, Haiping Hao, Kenneth W. Witwer & Norman J. Haughey. TNFα and IL-1β modify the miRNA cargo of astrocyte shed extracellular vesicles to regulate neurotrophic signaling in neurons. Cell Death & Disease, volume 9, Article number: 363 (2018), doi:10.1038/s41419-018-0369-4 Full text

Wu J, Tao Z, Deng Y, Liu Q1, Liu Y, Guan X, Wang X. Calcifying nanoparticles induce cytotoxicity mediated by ROS-JNK signaling pathways. Urolithiasis. 2018 Mar 6. doi: 10.1007/s00240-018-1048-8. [Epub ahead of print] PubMed

Jeon H, Yoo SM, Choi HS, Mun JY, Kang HG, Lee J, Park J, Gao SJ, Lee MS. Extracellular vesicles from KSHV-infected endothelial cells activate the complement system. Oncotarget. 2017 Oct 9;8(59):99841-99860. doi: 10.18632/oncotarget.21668. eCollection 2017 Nov 21. Full text

Jeon H, Yoo SM, Choi HS, Mun JY, Kang HG, Lee J, Park J, Gao SJ, Lee MS. Extracellular vesicles from KSHV-infected endothelial cells activate the complement system. Oncotarget. 2017 Oct 9;8(59):99841-99860. doi: 10.18632/oncotarget.21668. eCollection 2017 Nov 21. Full text

Ryan P. McNamara, Lindsey M. Costantini, T. Alix Myers, Blake Schouest, Nicholas J. Maness, Jack D. Griffith, Blossom A. Damania, Andrew G. MacLean, Dirk P. Dittmer. Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses, doi: 10.1128/mBio.02344-17, 6 February 2018 mBio vol. 9 no. 1 e02344-17 Full text

Yuting Liang, Longwei Qiao, Xia Peng, Zelin Cui, Yue Yin, Huanjin Liao, Min Jiang, Li Li. The chemokine receptor CCR1 is identified in mast cell-derived exosomes, Am J Transl Res 2018;10(2):352-367, 8; Published February 2018 Full text

Nasibeh Karimi, Aleksander Cvjetkovic, Su Chul Jang, Rossella Crescitelli, Mohammad Ali Hosseinpour Feizi, Rienk Nieuwland, Jan Lötvall, Cecilia Lässer. Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins, Cell. Mol. Life Sci. (2018). doi.org/10.1007/s00018-018-2773-4, First Online: 13 February 2018 Full text

Alexander J. Lakhter, Rachel E. Pratt, Rachel E. Moore, Kaitlin K. Doucette, Bernhard F. Maier, Linda A. Di Meglio, Emily K. Sims. Beta cell extracellular vesicle miR-21-5p cargo is increased in response to inflammatory cytokines and serves as a biomarker of type 1 diabetes, Diabetologia (2018). doi.org/10.1007/s00125-018-4559-5Full text

A. Weber, L. Cardone, S. S. Liu, A. L. Büttner, P. Rellecke, S. Sixt, A. Lichtenberg, P. Akhyari. Evaluation of Circulating Exosomes to Predict Emerging Valve Prosthesis-patient Mismatches after Surgical Aortic Valve Replacement, Thorac cardiovasc Surg 2018; 66(S 01): S1-S110, DOI: 10.1055/s-0038-1628013 Full text

A. Weber, L. Lageveen, Y. M. Lee, P. Rellecke, A. Assmann, S. Sixt, P. Horn, M. M. Cortese-Krott, A. Albert, A. Lichtenberg, P. Akhyari. Evaluation of Circulating Microparticles and Exosomes as Potential Biomarkers for Left Ventricular Hypertrophy in the Course of Aortic Valve Replacement, Thorac cardiovasc Surg 2018; 66(S 01): S1-S110, DOI: 10.1055/s-0038-1627992 Full text

Zhang, Q., Fu, L., Liang, Y., Guo, Z., Wang, L., Ma, C. and Wang, H. Exosomes originating from MSCs stimulated with TGF-β and IFN-γ promote Treg differentiation, Cell Physiol. Accepted Author Manuscript. doi:10.1002/jcp.26436, Accepted manuscript online: 16 January 2018 Abstract

Hanzi Xu, Zhen Gong, Yang Shen, Yichen Fang& Shanliang Zhong . Circular RNA expression in extracellular vesicles isolated from serum of patients with endometrial cancer, Epigenomics Vol. 10, No. 2 Research Article, Published Online: 15 Jan 2018 doi.org/10.2217/epi-2017-0109Full text

Katherine A. Ruppert, Tin T. Nguyen, Karthik S. Prabhakara, Naama E. Toledano Furman, Amit K. Srivastava, Matthew T. Harting, Charles S. Cox Jr. & Scott D. Olson. Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord Injury, Scientific Reports volume 8, Article number: 480 (2018), doi:10.1038/s41598-017-18867-w Published online: 11 January 2018 Full text

Peiyao Li, Jianbo Feng, Yang Liu, Qiang Liu, Li Fan, Qing Liu, Xiaoling She, Changhong Liu, Tao Liu, Chunhua Zhao, Wei Wang, Guiyuan Li and Minghua Wu. Novel Therapy for Glioblastoma Multiforme by Restoring LRRC4 in Tumor Cells: LRRC4 Inhibits Tumor-Infitrating Regulatory T Cells by Cytokine and Programmed Cell Death 1-Containing Exosomes, Original Research ARTICLE, Front. Immunol., 11 December 2017 doi.org/10.3389/fimmu.2017.01748Full text

Jennifer Klump MD, Ulrike Phillipp, Marie Follo PhD, Anna Eremin MSc, Hannes Lehmann MD, Sigrun Nestel, Nikolas von Bubnoff Prof. Dr., Irina Nazarenko PD . Extracellular vesicles or free circulating DNA: where to search for BRAF and cKIT mutations?, Nanomedicine: Nanotechnology, Biology and Medicine, Volume 14, Issue 3, April 2018, Pages 875-882 Abstract

Kathrin Oelschlägel, Jenny Pfeiffer, Annegret Potthoff. Imitating the Weathering of Microplastics in the Marine Environment, In: Cocca M., Di Pace E., Errico M., Gentile G., Montarsolo A., Mossotti R. (eds) Proceedings of the International Conference on Microplastic Pollution in the Mediterranean Sea. Springer Water. Springer, Cham Abstract

Vera A. Tang, Tyler M. Renner, Anna K. Fritzsche, Dylan Burger & Marc-André Langlois. Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry, Scientific Reports volume 7, Article number: 17769 (2017), doi:10.1038/s41598-017-18227-8, Published online: 19 December 2017 Full text

Huishan Guo, Maneka Chitiprolu, Luc Roncevic, Charlotte Javalet, Fiona J. Hemming, My Tran Trung, Lingrui Meng, Elyse Latreille, Christiano Tanese de Souza, Danielle McCulloch, R. Mitchell Baldwin, Rebecca Auer, Jocelyn Côté, Ryan Charles Russell, Rémy Sadoul, Derrick Gibbings. Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy, Developmental Cell, Volume 43, Issue 6, 18 December 2017, Pages 716-730.e7 Abstract

Kristina Svennerholm, Kyong-Su Park, Johannes Wikström, Cecilia Lässer, Rossella Crescitelli, Ganesh V. Shelke, Su Chul Jang, Shintaro Suzuki, Elga Bandeira, Charlotta S. Olofsson & Jan Lötvall. Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction, Scientific Reports volume 7, Article number: 17434 (2017), doi:10.1038/s41598-017-16363-9, Published online: 12 December 2017 Full text

JL Kang, J Park, SJ Gao, MS Lee. Extracellular vesicles from KSHV-infected endothelial cells activate the complement system, Oncotarget, 2017, Vol. 8, (No. 59), pp: 99841-99860 Full text

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Operating ZetaView®

11 Positions Scan


To capture many individual particles in the field of view, the ZetaView® optic scans a wide field of the cell channel. In addition, additional sample volume is automatically added by means of a precise pump.

Nanoparticle Tracking Analysis (NTA) with ZetaView®

Brownian Diffusion in the Laser Scattering Video Microscope

120 nm Al203 particles and agglomerates in the electrophoresis set-up of ZetaView

Particle Tracking Analysis (PTA) with ZetaView®

01.07.2014

Program
  • Theory
    • 90° light scattering set-up
    • Brownian motion hydrodynamic size, electrophoresis, zeta potential, concentration, size range, concentration range
       
  • Mechanical design
    • Scattering volume, minimum sample requirement
    • Minimum 1 sub-volume scan for truendess and high reproducibility of the results
    • Cell cassette design: Peltier, fluidics, easy cell change and cleaning
    • Lasers and filters
    • Light scattering and fluorescent mode
       
  • Software
    • Autoalignment
    • The importance of the correct camera settings
    • Statistical approach for the selection of acquired results
       
  • Application examples
    • Gold, silver
    • Liposomes, proteins
    • Clinical samples: Exosomes of various origin (tumor, aging, dermatology, cell culture, serum)
       
  • Limits and strengths of the ZetaView PTA
  • Complementarity with 180° DLS results
  • Final statement
  • Video of operation
  • Q&A