Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.

Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.

Very short measuring time:
With the Zetaview® you can measure more than 1000 particles within 90 seconds.

Robust statistics with outlier control
11 different positions within the measuring cell yield statistically robust results compared to only one measuring position. The implemented outlier control detects artifacts in the video quality and only considers flawless videos.

Save time by focusing the optics and during functional testing
For the "AutoAlignment" and the daily quality test, there is a "ready to use" 99 nm reference standard in the measurement concentration of 2.1x107 / ml, filled in a 3 ml syringe.

SOPs for pre-programmed work
Presets of measurement parameters such as size and surface charge are adjusted to scatter- or fluorescence mode, respectively.

Robust and contrast-optimized quartz glass measuring cell
The quartz glass makes the measuring cell durable and robust for cleaning procedures while maintaining high contrast for clear video.

Easy sample injection
The injection takes place with commercial syringes. The optimal measurement concentration is 2x107 particles / ml. Depending on the sample, typical dilutions are from 1:10 to 1: 10,000.

Sample recovery
The NTA measuring cell unit requires at least 600 μl sample. The sample can be recovered after the measurement without further effort,

ZetaView® fits in a pilot case and is easy to carry
That's what our sales and support people love, and of course our customers.

Fluorescence and photostability
The timing of the laser is optimized for minimal bleaching of low-lifetime fluorophores. Nevertheless, selecting stable fluorophores for the analysis of conjugated extracellular vesicles is advantageous. The ability to detect less than 1 fluorescent particle per 10,000 non-fluorescent increases significantly.

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Dr Genevieve Melling of Oxford Brookes University using the ZetaView® to study EVs

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11 Positions Scan


To capture many individual particles in the field of view, the ZetaView® optic scans a wide field of the cell channel. In addition, additional sample volume is automatically added by means of a precise pump.

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Brownian Diffusion in the Laser Scattering Video Microscope

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