Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.

Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.

The model PMX-120 BASIC is the platform, on which the other two are built on: PMX-220 TWIN and PMX-420 QUATT. Therefore, the series offers upgradeability making budgetary decisions easier. The benefits mentioned below apply for all three models.

 

ZetaView® NTA Simulator

Scanning NTA
Automated measurements taken at 11 distinct positions in the sample cell offer a more representative analysis than single-point measurements. Outlier control selects flawless videos. Autoalignment and Autofocus keep the video quality constant.

Multi-Parameter NTA
No other nanoparticle analysis method offers more analysis parameters per sample. At high throughput, in scattering as well as in fluorescent mode.

NTA size and NTA zeta potential are absolute measurands
Time consuming and costly calibration procedures do not apply.

Z-NTA Surface charge mirrors surface status of nanoparticles
Quantity and type of proteins in the surface of e.g. extracellular vesicles particles or chemical nature of the surface influence the surface charge. Easy to measure in ZetaView® by means of the electrophoretic mobility.

Fluorescence analysis
Fluorescence NTA provides specific information about subpopulations in the sample. A sensitive CMOS camera combined with high filtering capability and short illumination time – known as “low bleach performance” – provides ZetaView® with a high F-NTA sensitivity.

Fluorescence F-NTA AND surface charge Z-NTA in one
Both parameters, zeta potential and fluorescent light are dependent on the type of macromolecules or on the molecular composition of the particle surface.

Two cell assemblies
The standard "NTA" cell cassette is designed for high sample throughput, whereby each user can use his own sample cell. This is also suitable for measurements in the biological safety area.

In addition to all measurement parameters, zeta potential analyzes can be carried out with the Z-NTA cassette. Both cell assemblies can be quickly and easily replaced with a slide-in / slide-out and click mechanism.

High sample throughput

No high cost consumables
Just syringes and reference beads for scattered light and fluorescence measurements, if applicable. The life time of the quartz cell is several years. It is easy to clean with pipe cleaners.

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Operating ZetaView®

11 Positions Scan


To capture many individual particles in the field of view, the ZetaView® optic scans a wide field of the cell channel. In addition, additional sample volume is automatically added by means of a precise pump.

Nanoparticle Tracking Analysis (NTA) with ZetaView®

Brownian Diffusion in the Laser Scattering Video Microscope

120 nm Al203 particles and agglomerates in the electrophoresis set-up of ZetaView

Nanoparticle Tracking Analysis (NTA) with the Particle Metrix ZetaView® instrument family allows fast and reliable measurement of size, concentration, and Zeta Potential of nanoparticles. In contrast to scatter-based particle detection, fluorescence detection capability of the ZetaView® instrument allows the user to get biochemical information about particles. This increases the resolution and discriminatory power of the data. Furthermore, our recently introduced “Multi Filter Feature” allows Fluorescence Nanoparticle Tracking Analysis performing multi fluorescence with only one laser.  Thus, two different fluorescence dyes can be detected with one wavelength making F-NTA in the ZetaView® instrument more versatile ever since.

Get the newest updates of biomarker detection with NTA:

  • Release of multiple filter upgrade: Double staining with ONE laser!
  • Optimization of labeling strategies
  • Release of FDA 21 CFR part 11 software

Nanoparticle Tracking Analysis (NTA) with the Particle Metrix ZetaView® instrument family allows fast and reliable measurement of size, concentration, and Zeta Potential of nanoparticles in the size range from 10 nm to 2 µm. On basis of the scatter data, the user cannot recognize and distinguish whether the particles are biological vesicles, protein aggregates, inorganic precipitates or simply cell waste. However, the fluorescence detection capability of the ZetaView® instrument allows the user to get biochemical information about particles, thus increasing the resolution and discriminatory power of the data. In this video we describe the cutting-edge measurement technology of the ZetaView® instrument family and present the latest f-NTA results on extracellular vesicle phenotyping using membrane dyes and monoclonal antibodies.