ZetaView® NTA Nanoparticle Video Microscope
ZetaView®

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.

Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.

The model PMX-120 BASIC is the platform, on which the other two are built on: PMX-220 TWIN and PMX-420 QUATT. Therefore, the series offers upgradeability making budgetary decisions easier. The benefits mentioned below apply for all three models.

 

ZetaView® NTA Simulator

Scanning NTA
Automated measurements taken at 11 distinct positions in the sample cell offer a more representative analysis than single-point measurements. Outlier control selects flawless videos. Autoalignment and Autofocus keep the video quality constant.

Scanning 90° scattering microscope with video camera
Scanning 90° scattering microscope with video camera

Multi-Parameter NTA
No other nanoparticle analysis method offers more analysis parameters per sample. At high throughput, in scattering as well as in fluorescent mode.

Benefit_2

NTA size and NTA zeta potential are absolute measurands
Time consuming and costly calibration procedures do not apply.

Benefit_3
The mean square displacement of an individual particle – measured in about a second and inserted into the Stokes Einstein formula, delivers the particle size.

Z-NTA Surface charge mirrors surface status of nanoparticles
Quantity and type of proteins in the surface of e.g. extracellular vesicles particles or chemical nature of the surface influence the surface charge. Easy to measure in ZetaView® by means of the electrophoretic mobility.

Benefit_4
The electrophoretic mobility v / E is determined by applying an electric field E and measuring the resulting velocity v of the particles. For practical reasons, the Helmholtz zeta potential is derived from that.

Fluorescence analysis
Fluorescence NTA provides specific information about subpopulations in the sample. A sensitive CMOS camera combined with high filtering capability and short illumination time – known as “low bleach performance” – provides ZetaView® with a high F-NTA sensitivity.

Benefit_5
Excitiation of EV-antibody conjugates with a 488 nm laser. Green fluorecence filtered by a 500 nm LWP filter.

Fluorescence F-NTA AND surface charge Z-NTA in one
Both parameters, zeta potential and fluorescent light are dependent on the type of macromolecules or on the molecular composition of the particle surface.

Biologic nanoparticle with proteins of negative charge on the surface (pH=7).
Biologic nanoparticle with proteins of negative charge on the surface (pH=7).
Biologic nanoparticle with proteins and conjugated antibodies on the surface (pH=7).
Biologic nanoparticle with proteins and conjugated antibodies on the surface (pH=7).

Two cell assemblies
The standard "NTA" cell cassette is designed for high sample throughput, whereby each user can use his own sample cell. This is also suitable for measurements in the biological safety area.

In addition to all measurement parameters, zeta potential analyzes can be carried out with the Z-NTA cassette. Both cell assemblies can be quickly and easily replaced with a slide-in / slide-out and click mechanism.

« NTA » Cell assembly
« NTA » Cell assembly
« Z-NTA » Cell assembly
« Z-NTA » Cell assembly

High sample throughput

Benefit_8

No high cost consumables
Just syringes and reference beads for scattered light and fluorescence measurements, if applicable. The life time of the quartz cell is several years. It is easy to clean with pipe cleaners.

Cleaning of the “Z- NTA” cell channel
Cleaning of the “Z- NTA” cell channel
Cleaning of the “NTA” cell channel
Cleaning of the “NTA” cell channel

Sales inquiry

Your contact data
Fields with * are mandatory!

Operating ZetaView®

11 Positions Scan


To capture many individual particles in the field of view, the ZetaView® optic scans a wide field of the cell channel. In addition, additional sample volume is automatically added by means of a precise pump.

Nanoparticle Tracking Analysis (NTA) with ZetaView®

Brownian Diffusion in the Laser Scattering Video Microscope

120 nm Al203 particles and agglomerates in the electrophoresis set-up of ZetaView