Andreas Baur leads a group in the Department of Dermatology at the University Hospital of Erlangen. Their research focuses on the quantification of extracellular vesicles (EV) in plasma of patients. The group has used several different approaches as recently demonstrated in their paper published in EBioMedicine1 (03/2016). This reported on the quantification of EV-derived microRNA, the measurement of protein concentration in sucrose fractions that contain EV and the measurement of particles in sucrose gradient fractions. This work has been performed using the ZetaView system from Particle Metrix. Used together, these parameters provide a rough estimate on the relative concentration of EV in plasma.
The Baur group chose the ZetaView system for microparticle characterization because it is the only way to get actual numbers of nanoparticles in a given solution. All other measuring methods are semi-quantitative at best. However, it is advisable to consider particle measurements not as absolute numbers unless serial dilutions have been made and measured and different patient samples have been compared and, these again have to be in a comparable range of particle numbers.
Prior to using ZetaView, they did not use any EV quantification tool/device, but rather measured the concentrations indirectly as outlined above. As principle user of the system, Jochen Dindorf, says, “ZetaView is the only way to get actual particle numbers, rather than a semi-quantitative assessment of EV in a given solution, e.g. by western blot or RNA quantification. I also find it an easy instrument to teach new users how to use. This is really useful for our lab.”
ZetaView allows the measurement of quantitative information on the number of particles in a particular preparation as well as basic physical information (i.e. size and zeta potential). To receive more details about Particle Metrix and their family of particle characterization solutions, please contact Particle Metrix on +49 (0)2150-705679-0, visit the web site: www.Particle-Metrix.de.
- HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection: Lee et al, dx.doi.org/10.1016/j.ebiom.2016.03.004.
About Particle Metrix GmbH
Particle Metrix GmbH develops and manufactures instruments to characterize particles with a family of innovative analytical systems. Nanoparticle Tracking Analysis (Optical Particle Tracking), 180° dynamic light scattering and streaming potential charge mapping are employed to deliver answers on dispersion stability, zeta potential, charge density, particle size, molecular weight and concentration. Scattered and fluorescent light as well as electrical streaming potential signals are detected and analyzed to deliver rapid analyses for a broad range of materials.
The products are made in Germany and are distributed worldwide. These are backed with certification under DIN EN ISO 9001. A fast growing publications list from users in research and industry testify to the reliability and confidence of the product line and Particle Metrix’s competence to support them.
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