Analysis of 25 nm functionalized QDs using fluorescence-NTA with ZetaView® QUATT system
Application Note Download Abstract While it is uncertain how far the NTA community is able to push the limits of current technology in order to
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Particle Metrix, developers of versatile particle characterization solutions for the life sciences, report on how Cell Guidance Systems use the ZetaView® particle characterization system for exosome research and quality control.
Cell Guidance Systems (CellGS) provides innovative life science research tools and services. Their goal is to identify valuable innovations from research groups around the world and develop products that address unmet needs, providing new tools that expand the possibilities of life science research. The UK facility is located on the Babraham Science Park near Cambridge. Here, CellGS is developing novel tools and technologies for exosome research. In particular, they manufacture the Exo-spin range of exosome purification kits and TRIFic assay kits and are developing additional technologies to enable exosome research.
Exosomes are small lipid spheres around 40-150 nm in diameter that form within the cell. Exosomes contain and protect various RNA and protein cargos which are secreted into the blood and other bodily fluids. Exosomes are taken up by nearby and distant cells where their cargo can profoundly influence cellular activity. Consequently, there is interest in the potential of exosomes as vectors for gene therapy. In addition, many groups are now working on diagnostic products which can detect disease based on analysis of exosomes recovered from blood, urine, saliva and other body fluids.
For CellGS, developing new processes in research through to tight quality control procedures for production items requires the best in instrumentation. Speaking to this challenge, CellGS CEO, Michael Jones, describes their particle characterization needs and why they chose the Particle Metrix ZetaView® system to perform research into exosomes. “The ZetaView® enables our scientists to assess the quality of our exosome technologies and perform vital QC steps during manufacture. We selected ZetaView® after a full evaluation of competitive nanoparticle characterization systems. For us, the benefits in using the ZetaView® compared to other systems are its ease of use and throughput of sample measurement.”
The ZetaView® is a high performance integrated instrument for individual exosome particle tracking, classical micro-electrophoresis and Brownian motion. Auto-alignment and auto-focussing capabilities make the ZetaView® both fast and easy to use. A throughput of >20 samples per hour can readily be achieved. Highly resolved zeta potential and size histograms, as well as profiles, are routinely derived from thousands of particles. In addition, low concentrations of particles can be determined. The ZetaView® provides analysis certainty, leading to a better level of exosome characterization and enabling new discoveries in microvescicle and exosome research.
Application Note Download Abstract While it is uncertain how far the NTA community is able to push the limits of current technology in order to
After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.
In this new application note we describe a quick determination of the physical titer of coronaviruses by fluorescence-based Nanoparticle Tracking Analysis and specific spike antibody recognition.
In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.
In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive Pulse Sensing (MRPS) and the Single Particle Interference Reflectance Image Sensor (SP-IRIS) methods have been widely used as an alternative means of characterizing EVs. In this note, we relay two specific cases for using the ZetaView® NTA system to achieve relative “high-throughput” analysis of many EV samples, along estimations of throughput for MRPS & SPIRIS methods for an equivalent number of samples; further, we establish realistic estimates for the high cost of ownership for operating MRPS & SP-IRIS systems as a result of the cost of consumables as well as the substantially greater amount of time spent to run the same number of samples.
Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.
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